The OVS and OVA primer sequences were 5'-AGGCGGTGGAATGGA AAGA-3' and 5'-CCAGCAGGTATGCCAGGATG-3', respectively. PCR with OVS- and OVA-specific primers was performed as previously described. Dried scabs were collected from the infected goats and stored at -70℃ until analysis.ĭetails of the orf viruses isolated from Korean black goats Heavy scabs on the lips were observed in all the animals. The complete B2L and VIR genes were sequenced, and phylogenetic trees were subsequently constructed.įive suspected cases of contagious ecthyma in Korean black goats were reported in different geographic areas of Korea between 20 ( Table 1).

In the present study, five ORFVs were isolated from Korean black goats and characterized to evaluate the genetic relationships between these ORFVs and other strains of this virus. Infection with this virus in black goats had not been previously observed in Korea prior to the import of dairy goats from abroad. Īn outbreak of ORFV infection among dairy goats in 2009 was reported in Korea. The importance of ORFV has increased recently due to emergence of this virus in new territories as well as the occurrence of interspecies infection and reinfection of animals previously infected. Patients with zoonotic ORFV infection develop nodular and papillomatous lesions mainly on the hands, face, and mouth. Contagious ecthyma (contagious pustular dermatitis) is a highly contagious disease that primarily affects sheep, goats, and wild ruminants, and is characterized by the formation of papules, nodules, or vesicles that develop into thick crusts or heavy scabs on the lips, gingiva, and tongue. The major viral envelope protein B2L and virus interferon resistance (VIR) genes have been used to measure genetic variation among parapoxvirus since these genes are a highly conserved.

This group includes the orf virus (ORFV), pseudocowpox virus, and bovine papular stomatitis virus that infect cattle along with the parapoxvirus of red deer in New Zealand. Members of the genus Parapoxvirus from the Poxviridae family are oval, enveloped viruses containing a double strand (ds)DNA genome.

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